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NALT Concept Space
U.S. Department of Agriculture

NALT Full

Concept information

  1. Research, Technology, Methods
  2. methodology
  3. immunologic techniques
  4. precipitin tests
  5. chromatin immunoprecipitation
  1. Fields of Study
  2. immunology
  3. immunologic techniques
  4. precipitin tests
  5. chromatin immunoprecipitation

Término preferido

chromatin immunoprecipitation

Tipo

  • Topic

Definición

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    Fuente: Medical Subject Headings

    Tipo: Concept definition

Concepto genérico

Sinónimos

  • chromatin immunoprecipitation sequencing

En otras lenguas

URI

https://lod.nal.usda.gov/nalt/336371

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RDF/XML TURTLE JSON-LD Creado 19/2/20, última modificación 29/10/20