@prefix nalt: <https://lod.nal.usda.gov/nalt/> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix dc: <http://purl.org/dc/terms/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .
@prefix ns0: <https://lod.nal.usda.gov/naltv#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

nalt:9550
  skos:prefLabel "técnicas de laboratorio"@es, "laboratory techniques"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:narrower nalt:203622 .

nalt:203622_def
  rdf:value "Técnica electrofisiológica empleada para estudiar células, membranas celulares y, ocasionalmente, orgánulos aislados. Todos los métodos de patch-clamp dependen de un sello muy resistente colocado entre una micropipeta y una membrana, el cual suele ser obtenido mediante una ligera succión. Entre las cuatro variantes más comunes se incluyen los pinzamientos plano, de célula adherida, al revés (de adentro hacia afuera) y de toda la célula. Los métodos de patch-clamp se emplean comúnmente para el pinzamiento de voltaje, es decir, para controlar el voltaje a través de la membrana y medir el flujo de corriente; sin embargo, también se usan métodos de pinzamiento de corriente, en los que se controla la corriente y se mide el voltaje."@es, "An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used."@en ;
  dc:source "Medical Subject Headings 2013" ;
  a <https://lod.nal.usda.gov/naltv#Definition> .

<https://lod.nal.usda.gov/naltv#Topic>
  rdfs:label "Topic"@en ;
  a owl:Class .

nalt:203622
  skos:altLabel "patch-clamp techniques"@en, "voltage-clamp technique"@en ;
  a skos:Concept, <https://lod.nal.usda.gov/naltv#Topic> ;
  skos:definition nalt:203622_def ;
  ns0:marc001 "71056" ;
  skos:prefLabel "patch-clamp technique"@en, "técnica de patch-clamp"@es ;
  skos:broader nalt:9550 ;
  dc:modified "2013-12-06"^^xsd:date ;
  dc:created "2013-08-19"^^xsd:date ;
  skos:hiddenLabel "voltage clamp techniques"@en, "voltage-clamp techniques"@en ;
  skos:inScheme <https://lod.nal.usda.gov/nalt> .

<https://lod.nal.usda.gov/nalt>
  rdfs:label "NALT Full"@en ;
  a skos:ConceptScheme .