@prefix nalt: <https://lod.nal.usda.gov/nalt/> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix dc: <http://purl.org/dc/terms/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix ns0: <https://lod.nal.usda.gov/naltv#> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .

nalt:9550
  skos:prefLabel "técnicas de laboratorio"@es, "laboratory techniques"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:narrower nalt:23400 .

nalt:23400
  skos:prefLabel "sistema libre de células"@es, "cell free system"@en ;
  skos:broader nalt:20189, nalt:9550 ;
  a skos:Concept, <https://lod.nal.usda.gov/naltv#Topic> ;
  skos:hiddenLabel "cell free culture"@en, "cell free assay"@en, "cell-free-culture"@en, "cell-free assays"@en, "cell-free-system"@en, "cell-free culture"@en, "cell free assays"@en, "cell-free assay"@en ;
  dc:modified "2018-02-27"^^xsd:date ;
  skos:definition nalt:23400_def ;
  skos:altLabel "cellfree system"@en, "cell-free system"@en ;
  dc:created "2006-01-19"^^xsd:date ;
  ns0:marc001 "7361" ;
  skos:related nalt:271977 ;
  skos:inScheme <https://lod.nal.usda.gov/nalt> .

nalt:271977
  skos:prefLabel "síntesis de proteínas libre de células"@es, "cell-free protein synthesis"@en ;
  a ns0:Topic, skos:Concept ;
  skos:related nalt:23400 .

nalt:23400_def
  rdf:value "Extracto celular fraccionado que mantiene una función biológica. Una fracción subcelular aislada mediante ultracentrifugación u otra técnica de separación primero se debe aislar para que un proceso se pueda estudiar sin las demás reacciones colaterales complejas que se producen en una célula. En consecuencia, el sistema libre de células es muy utilizado en biología."@es, "A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology."@en ;
  dc:source "Medical Subject Headings 2012" ;
  a ns0:Definition .

ns0:Topic
  rdfs:label "Topic"@en ;
  a owl:Class .

nalt:20189
  skos:prefLabel "fracciones subcelulares"@es, "subcellular fractions"@en ;
  a ns0:Topic, skos:Concept ;
  skos:narrower nalt:23400 .

<https://lod.nal.usda.gov/nalt>
  rdfs:label "NALT Full"@en ;
  a skos:ConceptScheme .