@prefix nalt: <https://lod.nal.usda.gov/nalt/> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix dc: <http://purl.org/dc/terms/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix ns0: <https://lod.nal.usda.gov/naltv#> .

nalt:26951_def
  rdf:value "Técnicas de selección usada para identificar los genes que codifican las proteínas interactivas. Se usan variantes para evaluar la interacción compleja entre las proteínas y otras moléculas."@es, "Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions."@en ;
  dc:source "Medical Subject Headings 2012" ;
  a <https://lod.nal.usda.gov/naltv#Definition> .

<https://lod.nal.usda.gov/naltv#Topic>
  rdfs:label "Topic"@en ;
  a owl:Class .

nalt:26951
  dc:created "2006-01-19"^^xsd:date ;
  skos:hiddenLabel "yeast two hybrid method"@en, "yeast-two hybrid analysis"@en, "yeast-two hybrid screen"@en, "yeast two hybrid assays"@en, "yeast two hybrid system"@en, "yeast two hybrid analyses"@en, "yeast two-hybrid approach"@en, "yeast two hybrid analysis"@en, "yeast two-hybrid"@en, "two-hybrid screening"@en, "two-hybrid-system-techniques"@en, "yeast two hybrid methods"@en, "yeast two-hybrid assays"@en, "yeast hybrid assay"@en, "yeast two hybrid approach"@en, "yeast-two hybrid analyses"@en, "yeast two-hybrid assay"@en, "two-hybrid screen"@en, "yeast two-hybrid methods"@en, "yeast two-hybrid method"@en, "yeast two hybrid screen"@en, "yeast hybrid assays"@en, "yeast two hybrid screening"@en, "yeast two-hybrid system"@en, "yeast two hybrid assay"@en, "yeast two-hybrid approaches"@en ;
  a skos:Concept, <https://lod.nal.usda.gov/naltv#Topic> ;
  skos:inScheme <https://lod.nal.usda.gov/nalt> ;
  skos:definition nalt:26951_def ;
  ns0:marc001 "38745" ;
  skos:prefLabel "técnica de doble híbrido"@es, "two hybrid system techniques"@en ;
  skos:altLabel "yeast-two hybrid assay"@en, "two hybrid analysis"@en, "two-hybrid system"@en, "two hybrid assay"@en, "two hybrid screening"@en ;
  skos:broader nalt:26948 ;
  dc:modified "2017-03-27"^^xsd:date ;
  skos:related nalt:24642 .

nalt:24642
  skos:prefLabel "interacciones proteína-proteína"@es, "protein-protein interactions"@en ;
  a ns0:Topic, skos:Concept ;
  skos:related nalt:26951 .

<https://lod.nal.usda.gov/nalt>
  rdfs:label "NALT Full"@en ;
  a skos:ConceptScheme .

nalt:26948
  skos:prefLabel "molecular clonación"@es, "molecular cloning"@en ;
  a ns0:Topic, skos:Concept ;
  skos:narrower nalt:26951 .

