@prefix nalt: <https://lod.nal.usda.gov/nalt/> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix dc: <http://purl.org/dc/terms/> .
@prefix ns0: <https://lod.nal.usda.gov/naltv#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .

nalt:207911
  skos:prefLabel "reacción en cadena de la polimerasa cuantitativa"@es, "quantitative polymerase chain reaction"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:broader nalt:8124 .

nalt:197967
  skos:prefLabel "amplificación rápida de los extremos de ADNc"@es, "rapid amplification of cDNA ends"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:broader nalt:8124 .

<https://lod.nal.usda.gov/nalt>
  rdfs:label "NALT Full"@en ;
  a skos:ConceptScheme .

nalt:209544
  skos:prefLabel "hibridación del ácido nucleico"@es, "nucleic acid annealing"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:related nalt:8124 .

nalt:8124_def
  rdf:value "Método in vitro para producir grandes cantidades de fragmentos específicos de ADN o ARN de longitud y secuencia definidas a partir de pequeñas cantidades de secuencia flanqueantes del oligonucleótido (primers)."@es, "In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers)."@en ;
  dc:source "Medical Subject Headings" ;
  a <https://lod.nal.usda.gov/naltv#Definition> .

nalt:17409
  skos:prefLabel "técnicas y protocolos genéticos"@es, "genetic techniques and protocols"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:narrower nalt:8124 .

nalt:34216
  skos:prefLabel "cebadores de ADN"@es, "DNA primers"@en ;
  a <https://lod.nal.usda.gov/naltv#Chemical>, skos:Concept ;
  skos:related nalt:8124 .

nalt:312563
  skos:prefLabel "análisis de la curva de fusión"@es, "melting curve analysis"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:related nalt:8124 .

nalt:275963
  skos:prefLabel "amplificación diferencial de los alelos"@es, "allelic dropouts"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:related nalt:8124 .

nalt:59138
  skos:prefLabel "polimorfismo de repeticiones de secuencia simple"@es, "simple sequence length polymorphism"@en ;
  a <https://lod.nal.usda.gov/naltv#Topic>, skos:Concept ;
  skos:broader nalt:8124 .

nalt:8124
  skos:definition nalt:8124_def ;
  skos:related nalt:15977, nalt:275961, nalt:292387, nalt:312563, nalt:275963, nalt:343052, nalt:209544, nalt:34216 ;
  skos:hiddenLabel "repetitive element-PCR"@en, "PCR technique"@en, "PCR-analysed"@en, "tetra-primer amplification refractory mutation system-polymerase"@en, "repetitive element sequence-based PCR"@en, "nested-polymerase chain reaction"@en, "nested PCR"@en, "REP-PCR (repetitive element-polymerase chain reaction)"@en, "scorpion polymerase chain reaction"@en, "polymerase-chain-reaction"@en, "ERIC-PCR (enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction)"@en, "multiplex PCR"@en, "nested polymerase chain reaction"@en, "tetra amplification refractory mutation system polymerase chain reaction"@en, "polymerase chain reaction-denaturing"@en, "PCR-analysis"@en, "PCR techniques"@en, "tetra amplification refractory mutation-system polymerase chain reaction"@en, "array-PCR"@en, "scorpion-polymerase chain reaction"@en, "PCR analysis"@en, "T-ARMS-PCR"@en, "ARISA PCR"@en, "polymerase chain reaction - restriction"@en, "enterobacterial repetitive intergenic consensus sequence-pcr"@en, "polymerase chain reaction-based"@en, "sandwich IPCR"@en, "repetitive sequence-based polymerase chain reaction"@en, "ribosomal intergenic spacer analysis"@en, "nested-PCR"@en, "PCR-analyzed"@en, "TARMS-PCR"@en, "microsatellite primed polymerase chain reaction"@en, "T-ARMS PCR"@en, "repetitive element-polymerase chain reaction"@en, "polymerase chain reaction-restriction"@en, "PCR-based"@en, "array PCR"@en, "enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction"@en ;
  ns0:marc001 "29573" ;
  skos:narrower nalt:226519, nalt:196301, nalt:207911, nalt:59137, nalt:4815, nalt:59138, nalt:59136, nalt:200653, nalt:197967 ;
  a ns0:Topic, skos:Concept ;
  dc:created "2006-01-19"^^xsd:date ;
  skos:inScheme <https://lod.nal.usda.gov/nalt-core>, <https://lod.nal.usda.gov/nalt-awic>, <https://lod.nal.usda.gov/nalt> ;
  skos:altLabel "multiplex polymerase chain reaction"@en, "DNA amplification technique"@en, "DNA amplification (in vitro)"@en, "amplification (genetic technique)"@en, "RNA amplification (in vitro)"@en, "PCR (genetic technique)"@en, "gene amplification (technique)"@en ;
  skos:exactMatch <http://id.loc.gov/authorities/subjects/sh89002539>, <http://aims.fao.org/aos/agrovoc/c_34079>, <http://id.agrisemantics.org/gacs/C306>, <http://id.cabi.org/cabt/93070> ;
  skos:prefLabel "polymerase chain reaction"@en, "reacción en cadena de la polimerasa"@es ;
  skos:broader nalt:17409 ;
  dc:modified "2020-09-11"^^xsd:date .

nalt:59137
  skos:prefLabel "transcripción reversa y reacción de polimerasa en cadena"@es, "reverse transcriptase polymerase chain reaction"@en ;
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 .

nalt:275961
  skos:prefLabel "errores de genotipado"@es, "genotyping errors"@en ;
  a ns0:Topic, skos:Concept ;
  skos:related nalt:8124 .

nalt:226519
  skos:prefLabel "ensayo de amplificación de mutación desajustada"@es, "mismatch amplification mutation assay"@en ;
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 .

nalt:292387
  skos:prefLabel "reacción en cadena de la hibridación"@es, "hybridization chain reaction"@en ;
  a ns0:Topic, skos:Concept ;
  skos:related nalt:8124 .

nalt:4815
  skos:prefLabel "polimorfismo de longitud de fragmentos amplificados"@es, "amplified fragment length polymorphism"@en ;
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 .

ns0:Topic
  rdfs:label "Topic"@en ;
  a owl:Class .

nalt:59136
  skos:prefLabel "técnica de ADN polimórfico amplificado al azar"@es, "random amplified polymorphic DNA technique"@en ;
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 .

nalt:15977
  skos:prefLabel "secuencias de nucleótidos"@es, "nucleotide sequences"@en ;
  a ns0:Topic, skos:Concept ;
  skos:related nalt:8124 .

nalt:196301
  skos:prefLabel "polimorfismos para la amplificación de regiones blanco"@es, "target region amplification polymorphism"@en ;
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 .

nalt:200653
  skos:prefLabel "paseo genómico"@es, "genome walking"@en ;
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 .

nalt:343052
  skos:prefLabel "amplificación de recombinasa polimerasa"@es, "recombinase polymerase amplification"@en ;
  a ns0:Topic, skos:Concept ;
  skos:related nalt:8124 .